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Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

Price and availability

526 012 ₸

Availability

Order now and get it on Friday April 02, 2021

Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15957-37] to NDUFS6 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free
    See all NDUFS6 primary antibodies
  • Description

    Rabbit monoclonal [EPR15957-37] to NDUFS6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251211 is the carrier-free version of ab195808. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15957-37
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Images

  • Flow Cytometry - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Flow Cytometry - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of NIH 3T3 cells (paraformaldehyde-fixed, 2%) labeling NDUFS6 with ab195808 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
  • Immunocytochemistry - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Immunocytochemistry - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HeLa cells (100% methanol-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFS6 with ab195808 at 1/200 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).Note: Cytoplasm staining on HeLa cell line was observed.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    This data was developed using ab195808, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFS6 with ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human kidney tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution + PC-12 cell lysate

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 3 minutes


    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 10 seconds


    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human skeletal muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 30 seconds


    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    All lanes : Anti-NDUFS6 antibody [EPR15957-37] (ab195808) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa


    Exposure time: 3 minutes


    This data was developed using ab195808, the same antibody clone in a different buffer formulation.

     

  • Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)
    Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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