Anti-NKp30 antibody [EPR14509] - BSA and Azide free (ab250880)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14509] to NKp30 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-NKp30 antibody [EPR14509] - BSA and Azide free
See all NKp30 primary antibodies -
Description
Rabbit monoclonal [EPR14509] to NKp30 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250880 is the carrier-free version of ab186425. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250880 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR14509 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : NCI-H460 lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaThis data was developed using ab186425, the same antibody clone in a different buffer formulation.
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Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution + K562 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDaThis data was developed using ab186425, the same antibody clone in a different buffer formulation.
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This data was developed using ab186425, the same antibody clone in a different buffer formulation.Western blot analysis of NK-p30 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.
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