Anti-Tim 2 antibody [RMT2-14] (ab281909)
![Anti-Tim 2 antibody [RMT2-14] (ab281909)](https://www.abcam.com/ps/products/281/ab281909/Images/ab281909-1-anti-TIM2-antibody-immunohistochemistry-splenocytes-mouse.jpg "Anti-Tim 2 antibody [RMT2-14] (ab281909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [RMT2-14] to Tim 2
- Suitable for: ICC
- Reacts with: Mouse
Overview
-
Product name
Anti-Tim 2 antibody [RMT2-14]
See all Tim 2 primary antibodies -
Description
Rat monoclonal [RMT2-14] to Tim 2 -
Host species
Rat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse Tim 2 aa 1-250 (extracellular). Raised by immunising Sprague Dawley rats with TIM-2-Ig extracellular domain and the Fc portion of mouse IgG2a.
Database link: Q8R183 -
Positive control
- ICC: Mouse splenocytes cells.
-
General notes
ab281909 is the recombinant rat version of ab281910.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RMT2-14 -
Isotype
IgG2a -
Light chain type
lambda -
Research areas
Images
-
Immunocytochemistry - (ab281909)
This data was developed using the chimeric version (ab281910).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix® coverslips stained with the chimeric ab281910 at 10 µg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. Mouse splenocytes were immobilized on Shi-fix® coverslips.
