Anti-Histone H2B (formyl K116) antibody (ab235424)
Key features and details
- Rabbit polyclonal to Histone H2B (formyl K116)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H2B (formyl K116) antibody
See all Histone H2B primary antibodies -
Description
Rabbit polyclonal to Histone H2B (formyl K116) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Histone H2B (formyl K116).
Database link: P62807 -
Positive control
- ICC/IF: HeLa cells (treated with 30mM sodium butyrate for 4hr). WB: HEK-293, A549, K562 and Jurkat whole cell lysates, treated (+) with 30mM sodium butyrate for 4hr.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H2B (formyl K116) antibody (ab235424)All lanes : Anti-Histone H2B (formyl K116) antibody (ab235424) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) (Treated with 30 mM sodium butyrate for 4 hours) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) (Treated with 30 mM sodium butyrate for 4 hours) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) (Treated with 30 mM sodium butyrate for 4 hours) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) (Treated with 30 mM sodium butyrate for 4 hours) whole cell lysate
Lane 5 : Non-treated HEK-293 whole cell lysate
Lane 6 : Non-treated A549 whole cell lysate
Lane 7 : Non-treated K562 whole cell lysate
Lane 8 : Non-treated Jurkat whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (formyl K116) antibody (ab235424)HeLa (human epithelial cell line from cervix adenocarcinoma) (Treated with 30 mM sodium butyrate for 4 hours) cells stained for Histone H2B (formyl K116) using ab235424 at 1/50 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
