Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GRO gamma antibody [EPR21759-11] (ab220431)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21759-11] to GRO gamma
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat

Product name

Anti-GRO gamma antibody [EPR21759-11]
See all GRO gamma primary antibodies
Description

Rabbit monoclonal [EPR21759-11] to GRO gamma
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat CINC-2 recombinant protein (aa33-100); Rat lung primary cell supernatant; RAW264.7 treated with LPS for 6 hours, with BFA added after 3 hours, whole cell lysate. ICC/IF: RAW 264.7 cells treated with LPS for 6 hours, with BFA added after 3 hours. Flow Cyt: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with LPS for 6 hours, with BFA added after 3 hours. IP: RAW264.7 treated with LPS for 6 hours, with BFA(added after 3 hours, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21759-11
Isotype

IgG
Research areas

Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

Lane 1 : Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300ng/ml Brefeldin A (BFA) added after 3 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression of CINC-2 increased LPS treatment of macrophages (PMID: 9576061).

Note: CINC-2 and GRO gamma are alternative names for the same target.
Immunoprecipitation - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

GRO gamma was immunoprecipitated from 0.35 mg of RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate with ab220431 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220431 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate 10 µg (Input).

Lane 2: ab220431 IP in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220431 in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Flow Cytometry - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours (Right panel) or untreated (Left panel) labeling GRO gamma with ab220431 at 1/500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Note: CINC-2 and GRO gamma are alternative names for the same target.
Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

Lane 1 : Rat CINC-2 recombinant protein (aa33-100) at 0.01 µg
Lane 2 : Rat lung primary cell supernatant at 10 µl

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 11 kDa

Exposure time : Lane 1: 15 seconds; Lane 2: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

Observed MW : 9 kDa (lane 1), 11 kDa (lane 2).

The molecular mass observed is consistent with what has been described in the literature (PMID: 12508094).

Note: CINC-2 and GRO gamma are alternative names for the same target.
Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours or untreated labeling GRO gamma with ab220431 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-GRO gamma antibody [EPR21759-11] (ab220431)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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