Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRO gamma antibody [EPR18170-142] (ab189486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18170-142] to GRO gamma
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GRO gamma antibody [EPR18170-142]
    See all GRO gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR18170-142] to GRO gamma

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat lung treated with lipopolysaccharides (LPS) culture supernatant. IP: Mouse lung treated with lipopolysaccharides (LPS) culture supernatant.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18170-142

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution

    Lane 1 : Untreated mouse lung culture supernatant
    Lane 2 : Mouse lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    The culture supernatant was collected from a large culture dish and it was not concentrated.

  • Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] (ab189486)

    GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
    Lane 2: ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    Western blot - Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution

    Lane 1 : Untreated rat lung culture supernatant
    Lane 2 : Rat lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa


    Exposure time: 6 seconds


    Blocking and dilution buffer/ concentration: 5% NFDM/TBST.

    The culture supernatant was collected from a large culture dish and it was not concentrated.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Anti-GRO gamma antibody [EPR18170-142] (ab189486)
    Anti-GRO gamma antibody [EPR18170-142] (ab189486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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