Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
![Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-321746-anti-gro-gamma-antibody-epr18170-142-immunoprecipitation.jpg "Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free
See all GRO gamma primary antibodies -
Description
Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung treated with lipopolysaccharides (LPS) culture supernatant.
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General notes
Ab229376 is the carrier-free version of ab189486. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab229376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18170-142 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
Lane 2: ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
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![Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-295228-anti-gro-gamma-antibody-epr18170-142-western-blot.jpg)
Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution
Lane 1 : Untreated rat lung culture supernatant
Lane 2 : Rat lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Exposure time: 6 secondsBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
The culture supernatant was collected from a large culture dish and it was not concentrated.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
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![Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-3-benefits-of-recombinant-antibodies.png)
Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
