Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free
    See all GRO gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat lung treated with lipopolysaccharides (LPS) culture supernatant.

  • General notes

    Ab229376 is the carrier-free version of ab189486. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18170-142

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
    Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)

    GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
    Lane 2: ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).

  • Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
    Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
    All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution

    Lane 1 : Untreated rat lung culture supernatant
    Lane 2 : Rat lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa


    Exposure time: 6 seconds


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    The culture supernatant was collected from a large culture dish and it was not concentrated.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).

  • Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
    Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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