Anti-ATP6V1A antibody (ab192355)
Key features and details
- Rabbit polyclonal to ATP6V1A
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ATP6V1A antibody
See all ATP6V1A primary antibodies -
Description
Rabbit polyclonal to ATP6V1A -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Raji, HepG2, C6, PC-12 and NIH/3T3 cell lysates; Human fetal brain and fetal kidney lysates; Mouse and rat brain lysates. IP: Mouse brain lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ATP6V1A antibody (ab192355) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-ATP6V1A antibody (ab192355) at 1/2000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-ATP6V1A antibody (ab192355) at 1/500 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-ATP6V1A antibody (ab192355) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cells) cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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ATP6V1A was immunoprecipitated from 1mg of Mouse brain lysate with ab192355 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab192355 at 1/500 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse brain lysate 10 µg (Input). Lane 2: ab192355 IP in Mouse brain lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192355 in Mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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ATP6V1A was immunoprecipitated from 1mg of Mouse brain lysate with ab192355 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab103445 at 1/200 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse brain lysate 10 µg (Input). Lane 2: ab192355 IP in Mouse brain lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192355 in Mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
ab192355 was used to Immunoprecipitate ATPV1A. These precipitates were resolved with ab103445.