Anti-PDCD10/CCM3 antibody (ab226181)
Key features and details
- Rabbit polyclonal to PDCD10/CCM3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDCD10/CCM3 antibody
See all PDCD10/CCM3 primary antibodies -
Description
Rabbit polyclonal to PDCD10/CCM3 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human PDCD10/CCM3 aa 75-125. The exact sequence is proprietary.
Database link: Q9BUL8 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab226181 has not performed satisfactorily when used for WB of PDCD10/CCM3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PDCD10/CCM3.
This product was previously labelled as PDCD10
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226181 was affinity purified using an epitope specific to PDCD10/CCM3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-PDCD10/CCM3 antibody (ab226181)
PDCD10/CCM3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226181 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226181 at 0.4 µg/ml.
Lane 1: ab226181 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
