Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358]
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293-T, HepG2, Jurkat, and MCF-7 cell lysates. Flow Cyt: Jurkat cells.

  • General notes

    This product was previously labelled as Phospholipase C gamma 1. 

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PLCG1 knockout HEK-293T cell lysate

    Lysates/proteins at 40 µg/ml per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 149 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

    ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Phospholipase C gamma 1 / PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HepG2 cell lysate (20 µg)
    Lane 4: Mouse brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109501 was shown to recognize Phospholipase C gamma 1 / PLC-gamma-1 when Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab109501 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 149 kDa

  • Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Flow Cytometry - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Flow cytometric analysis of permeabilized Jurkat cells using ab109501 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
  • Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
    Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)

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