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Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y]
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Host species

    Rabbit
  • Specificity

    ab76031 detects Phospholipase C gamma 1/PLC-gamma-1 phosphorylated at Y783.

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blotmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.

  • Positive control

    • WB: Jurkat cell lysates treated with pervanadate. ICC/IF: Jurkat cells treated with pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C gamma 1.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1898Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate
    Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate
    Lane 3 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    Dot Blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

    Dot blot analysis of Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) phospho peptide (Lane 1) and Phospholipase C gamma 1/PLC-gamma-1 non-phospho peptide (Lane 2) labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/200000 dilution

    Lane 1 : Jurkat cell lysates, untreated
    Lane 2 : Jurkat cell lysates, treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 149 kDa
    Observed band size: 149 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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