Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
Key features and details
- Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) -
Host species
Rabbit -
Specificity
ab76031 detects Phospholipase C gamma 1/PLC-gamma-1 phosphorylated at Y783.
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Tested applications
Suitable for: WB, ICC/IF, Dot blotmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
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Positive control
- WB: Jurkat cell lysates treated with pervanadate. ICC/IF: Jurkat cells treated with pervanadate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C gamma 1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1898Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) phospho peptide (Lane 1) and Phospholipase C gamma 1/PLC-gamma-1 non-phospho peptide (Lane 2) labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/200000 dilution
Lane 1 : Jurkat cell lysates, untreated
Lane 2 : Jurkat cell lysates, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDa