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Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y]
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Host species

    Rabbit
  • Specificity

    This antibody detects Phospholipase C gamma 1/PLC-gamma-1 phosphorylated on Tyrosine 783.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783).
    Database link: P19174

  • Positive control

    • WB: Jurkat cell lysate treated with Pervanadate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C gamma 1.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2611Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis

Images

  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)
    Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)
    All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    untreated
    Lane 2 : Jurkat cell lysate
    treated with Pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 148 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)
    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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