Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (ab41434)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) -
Host species
Rabbit -
Specificity
Phospho-Tyr775-\Phospholipase C gamma 1/PLC-gamma-1 specific. Immunogen sequence has low homology to Phospholipase C gamma 2.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
-
Positive control
- Human Jurkat and A431 cells treated with pervanadate.
-
General notes
Do not aliquot.Previously labelled as Phospholipase C gamma 1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide, then affinity purified using phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr775) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody (ab41434)
Western blot analysis of Phospholipase C gamma 1/PLC-gamma-1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-Phospholipase C gamma 1/PLC-gamma-1. The blots were probed with anti- Phospholipase C gamma 1/PLC-gamma-1 (lanes 1 & 2) and anti-Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
