Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)
![Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)](https://www.abcam.com/ps/products/251/ab251453/Images/ab251453-3-anti-icam1-antibody-epr19938-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19938] to ICAM1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Rat
Overview
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Product name
Anti-ICAM1 antibody [EPR19938] - BSA and Azide free
See all ICAM1 primary antibodies -
Description
Rabbit monoclonal [EPR19938] to ICAM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251453 is the carrier-free version of ab206398. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251453 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19938 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)This data was developed using ab206398, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on sinusoidal endothelial cells of rat liver [PMID: 17058232]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)](https://www.abcam.com/ps/products/251/ab251453/Images/ab251453-2-anti-icam1-antibody-epr19938-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)This data was developed using ab206398, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ICAM1 with ab206398 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on extracellular matrix and blood vessels of rat kidney [PMID: 9198039] [PMID 7902311]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)](https://www.abcam.com/ps/products/251/ab251453/Images/ab251453-399395-anti-icam1-antibody-epr19938-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)All lanes : Anti-ICAM1 antibody [EPR19938] (ab206398) at 1/5000 dilution
Lane 1 : Rat spleen lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 4 : L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg
Lane 5 : Rat liver lysate at 20 µg
Lane 6 : Rat kidney lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?This data was developed using ab206398, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 5 seconds; Lanes 5-6: 1 minute.
The observed molecular weight is consistent with the literature (PMID: 1712683).
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![Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)](https://www.abcam.com/ps/products/251/ab251453/Images/ab251453-4-benefits-of-recombinant-antibodies.png)
Anti-ICAM1 antibody [EPR19938] - BSA and Azide free (ab251453)
