Anti-HE4 antibody (ab273130)
Key features and details
- Rabbit polyclonal to HE4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HE4 antibody
See all HE4 primary antibodies -
Description
Rabbit polyclonal to HE4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HE4 aa 1 to the C-terminus. Mature chain.
Database link: Q14508 -
Positive control
- IHC-P: Human cervix, uterine tissue. WB: EFO-21 cell lysate. ICC-IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HE4 antibody (ab273130)
PFA-fixed, Triton X-100-permeabilized A431 (Human epidermoid carcinoma cell line) cells stained for HE4 using ab273130 at 2 µg/ml (green) in ICC/IF.
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Western blot - Anti-HE4 antibody (ab273130)Anti-HE4 antibody (ab273130) at 0.4 µg/ml + EFO-21 (Human cell line) cell lysate
Predicted band size: 13 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody (ab273130)Paraffin-embedded human cervix (uterine) tissue stained for HE4 using ab273130 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HE4 antibody (ab273130)Paraffin-embedded human ovary tissue stained for HE4 using ab273130 at 1/50 dilution in immunohistochemical analysis.
Shows low expression as expected.
Retrieval method: HIER pH6.