Astana Biomed Group, an authorized Abcam distributor in Central Asia

FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)

Key features and details

  • FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
    See all alpha Tubulin primary antibodies

  • Description

    FITC Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Gerbil

  • Immunogen

    Full length native protein (purified) corresponding to alpha Tubulin.

  • Epitope

    aa426-450. Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin.

  • Positive control

    • ICC/IF: HeLa cells. IHC-Fr: Xenopus laevis stage 36 tissue. Flow cyt: HeLa cells. Cultured human fibroblasts, baby hamster kidney (BHK) cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
    ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Flow Cytometry - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
    Flow Cytometry - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
    Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

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