Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free
    See all Glutathione Peroxidase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247669 is the carrier-free version of ab108429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247669 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)

    This data was developed using ab108429, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)

    Lane 3: THP1 cell lysate (20 µg)

    Lane 4: HL60 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    Flow Cytometry - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    This data was developed using ab108429, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of THP-1 (human acute monocytic leukemia ) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    Western blot - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa



    This data was developed using ab108429, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    This data was developed using ab108429, the same antibody clone in a different buffer formulation.ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)
    Anti-Glutathione Peroxidase 1 antibody [EPR3311] - BSA and Azide free (ab247669)

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