Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab90718)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 aa 750-850 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104209) -
Positive control
- This antibody gave a positive signal in SW480 Whole Cell Lysate.
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General notes
Previously labelled as Phospholipase C gamma 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 149 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 149 kDa).Target
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Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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Database links
- Entrez Gene: 281987 Cow
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P08487 Cow
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab90718)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab90718) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 Whole Cell Lysate with Immunising Peptide at 1 µg/ml
Lane 3 : SW480 Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 149 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Datasheets and documents
References (1)
ab90718 has been referenced in 1 publication.
- Ma T et al. Activation of brain-derived neurotrophic factor signaling in the basal forebrain reverses acute sleep deprivation-induced fear memory impairments. Brain Behav 10:e01592 (2020). PubMed: 32157827
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab90718)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab90718) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 Whole Cell Lysate with Immunising Peptide at 1 µg/ml
Lane 3 : SW480 Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 149 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
