Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸ 10 µl 127 315 ₸

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Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [NIAR164] to Mitofusin 2
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Mitofusin 2 antibody [NIAR164]
See all Mitofusin 2 primary antibodies
Description

Rabbit monoclonal [NIAR164] to Mitofusin 2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human Mitofusin 2. The exact sequence is proprietary.
Database link: O95140
Positive control
- WB: Mouse brain lysate, mouse kidney lysate, rat brain lysate, HeLa cell lysate, Jurkat cell lysate, HEK293 cell lysate, Raji cell lysate, rat primary neurons cell lysate. ICC/IF: HEK293 cells. IHC-P: Human kidney tissue.
General notes
This Mitofusin 2 antibody (ab124773) was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

NIAR164
Isotype

IgG
Research areas

Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

Lane 1 : mouse brain lysate
Lane 2 : mouse kidney lysate
Lane 3 : rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773) This image is courtesy of an anonymous Abreview

Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg

Secondary
Anti-rabbit IgG HRP conjugate at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 86 kDa

Exposure time: 30 seconds
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

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