Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total Phospholipase C gamma 1/PLC-gamma-1 protein.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey
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Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
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Positive control
- Human breast carcinoma tissue (IHC-P).
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General notes
Previously labelled as Phospholipase C gamma 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200)
Paraffin-embedded human breast carcinoma tissue stained for Phospholipase C gamma 1/PLC-gamma-1, using ab52200 (1/100). The right hand panel represents a negative control where ab52200 was pre-incubated with the immunizing (blocking) peptide.
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min).
Lane 2 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min). For this panel, ab52200 was pre-icubated with the immunizing (blocking) peptide.
Predicted band size: 149 kDa
Observed band size: 149 kDa
