Anti-PLCG 2 (phospho Y753) antibody (ab75659)
Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y753)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCG 2 (phospho Y753) antibody
See all PLCG 2 primary antibodies -
Description
Rabbit polyclonal to PLCG 2 (phospho Y753) -
Host species
Rabbit -
Specificity
ab75659 detects endogenous levels of PLCG 2 only when phosphorylated at tyrosine 753. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human PLCG 2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659)All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution
Lane 1 : A431 cell extracts, untreated
Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)
Predicted band size: 148 kDa
Observed band size: 148 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (ab75659)ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.
