Native KLH protein (Low endotoxin) (ab157012)
Key features and details
- Expression system: Native
- Purity: > 90% Size exclusion
- Endotoxin level:
- Suitable for: Conjugation
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Product name
Native KLH protein (Low endotoxin) -
Purity
> 90 % Size exclusion.
ab157012 was determined to be >90% pure as a mixture of high and low molecular weight forms and >50% pure as high molecular weight forms by size exclusion chromatography. -
Endotoxin level
Expression system
NativeAccession
Protein length
Full length proteinAnimal free
NoNature
Native-
Species
Megathura crenulata -
Sequence
DFGHSKKIRK NVHSLTAEEQ NSLRRAMDDL QDDKTRGGFQ QIAAFHGEPK WCPRPEAEKK FACCVHGMAV FPHWHRLLTV QGENALRKHG FTGGLPYWDW TRPMSALPHF VADPTYDDSV SSLEEDNPYS HGHIDSVGHD TTRAVRDDLY QSPGFGHYTD IAKQVLLALE QDDFCDFEVQ FEIAHNSIHA LVGGNEPYGM STLEYFLYDP IFFLHHSNTD RLWAIWQALQ KYRGKPYNTA NCAIVRHDTY RKPLQPFGLD SVINPDDETR EHSVPRDVFN YKDDFNYEYE SLNFNGLSIA QLDRELQRIK SHDRVFAGFL LHEIGQSALV KFYVCKHHVS DCDHYAGEFY ILGDEAEMPF AYDRVYKYEI SQALHDLDLH VGDNFHLKYE AFNLNGGSLG GVDLSQPSVI FEPAAGSHTA -
Additional sequence information
Molecular Weight >1 MDa
Description
Native KLH proteinAssociated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Conjugation
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Form
Lyophilized -
Additional notes
This preparation of chromatographically purified hemocyanin has been treated to reduce endotoxins for immunization protocols. Upon reconstitution with PBS a highly soluble solution is obtained for efficient and direct use for peptide coupling. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Lyophilized from BES buffer containing MgSO4.
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ReconstitutionSoluble in PBS or other physiological buffer at 5-10 mg/ml. Note that material is supplied as gross weight. Protein content is >60%
General Info
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Alternative names
- Hemocyanin 2 c chain
- Hemocyanin 2c chain
- Hemocyanin A chain
see all -
Relevance
KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits. -
Cellular localization
Secreted protein; extracellular space
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab157012 has not yet been referenced specifically in any publications.
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Lyophilized from BES buffer containing MgSO4.
-
ReconstitutionSoluble in PBS or other physiological buffer at 5-10 mg/ml. Note that material is supplied as gross weight. Protein content is >60%
