Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

Key features and details

  • Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]
    See all PLCG 2 primary antibodies

  • Description

    Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)

  • Host species

    Rabbit

  • Specificity

    ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).

  • Positive control

    • WB: Ramos and Raji cell lysates treated with pervanadate. IHC-P: Human lymph node tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) This image is courtesy of an Abreview submitted by Rudolf Jung

    ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
    Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

    Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ab52610 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
    All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

    Lane 1 : Raji cell lysate (untreated)
    Lane 2 : Raji cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 150 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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