Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
![Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)](https://www.abcam.com/ps/products/52/ab52610/Images/ab52610-248164-ab52610ihcp.jpg "Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)")
Key features and details
- Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]
See all PLCG 2 primary antibodies -
Description
Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217) -
Host species
Rabbit -
Specificity
ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217. -
Tested applications
Suitable for: WB, IHC-P, Dot blotmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).
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Positive control
- WB: Ramos and Raji cell lysates treated with pervanadate. IHC-P: Human lymph node tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1404Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) This image is courtesy of an Abreview submitted by Rudolf Jung
ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.
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![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)](https://www.abcam.com/ps/products/52/ab52610/Images/ab52610-5-ab52610WB1.jpg)
Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)](https://www.abcam.com/ps/products/52/ab52610/Images/ab52610-4-ab52610Dot1.jpg)
Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)](https://www.abcam.com/ps/products/52/ab52610/Images/ab52610ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ab52610 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)](https://www.abcam.com/ps/products/52/ab52610/Images/ab52610wb.jpg)
Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution
Lane 1 : Raji cell lysate (untreated)
Lane 2 : Raji cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 150 kDa
