Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)
![Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.com/ps/products/51/ab51020/Images/ab51020-62852-anti-plcg-2-phospho-y759-antibody-ep1406y-western-blot.jpg "Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)")
Key features and details
- Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]
See all PLCG 2 primary antibodies -
Description
Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759) -
Host species
Rabbit -
Specificity
This antibody only detects PLCG 2 phosphorylated on Tyrosine 759. -
Tested applications
Suitable for: ICC, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phospho peptide corresponding to residues surrounding Tyr759 of human PLCG 2.
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Positive control
- Raji cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1406Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)All lanes : Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution
Lane 1 : Raji cell lysate, untreated
Lane 2 : Raji cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.com/ps/products/51/ab51020/Images/ab51020-2-anti-plcg-2-antibody-ep1406y-immunocytochemistry-raji-human.jpg)
Immunocytochemistry - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
