Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12668] to KDEL - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat

Overview

  • Product name

    Anti-KDEL antibody [EPR12668] - BSA and Azide free
    See all KDEL primary antibodies

  • Description

    Rabbit monoclonal [EPR12668] to KDEL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240190 is the carrier-free version of ab176333. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240190 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling KDEL with purified ab176333 at 1/200. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    For negative control 1, ab150120 (Rabbit primary antibody) and anti-mouse secondary antibody were used and for negative control 2, ab7291 (Mouse primary antibody) and ab150077 (anti-rabbit secondary antibody) were used. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Flow Cytometry - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Flow Cytometry - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling KDEL (red) with purified ab176333 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunohistochemical analysis of paraffin-embedded rat kidney sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunohistochemical analysis of paraffin-embedded mouse liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunohistochemical analysis of paraffin-embedded human liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Flow Cytometry - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Flow Cytometry - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Flow cytometric analysis of permeabilized HeLa cells labeling KDEL with ab176333 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunofluorescent staining of MCF7 cells labeling KDEL ER with ab176333 at 1/100 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KDEL with ab176333 at 1/50 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling KDEL with ab176333 at 1/50 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176333).

  • Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
    Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)

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