Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-KDEL antibody [EPR12668] (ab176333)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12668] to KDEL
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Species independent

Product name

Anti-KDEL antibody [EPR12668]
See all KDEL primary antibodies
Description

Rabbit monoclonal [EPR12668] to KDEL
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293T, MCF-7, Raji, HepG2, HeLa, Jurkat, and K562 cell lysates. MCF7 and HeLa cells. Paraffin-embedded Human heart and kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.06% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12668
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [EPR12668] (ab176333)

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling KDEL with purified ab176333 at 1/200. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

For negative control 1, ab150120 (Rabbit primary antibody) and anti-mouse secondary antibody were used and for negative control 2, ab7291 (Mouse primary antibody) and ab150077 (anti-rabbit secondary antibody) were used.
Western blot - Anti-KDEL antibody [EPR12668] (ab176333)

All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 25 kDa
Western blot - Anti-KDEL antibody [EPR12668] (ab176333)

Lane 1 : Anti-P4HB antibody [EPR9498] (ab137119) at 1/5000 dilution
Lanes 2 & 4 & 6 & 8 & 10 : Anti-KDEL antibody [EPR12668] (ab176333) at 1/5000 dilution
Lane 3 : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution
Lane 5 : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/5000 dilution
Lane 7 : Anti-ERp57 antibody [EPR10678(B)] (ab154191) at 1/5000 dilution
Lane 9 : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/5000 dilution
Lane 11 : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/5000 dilution

All lanes : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 25 kDa

Exposure time: 3 minutes

ab176333 detected several bands which have similar MWs with some proteins containing carboxyl terminal KDEL motifs, including P4HB, GRP78 BiP, GRP94, PDIA6 and LEPRE1 (PMID: 25683117, PMID: 19741001, PMID: 22079671, PMID: 28648146, PMID: 22615817). It indicates that ab176333 might recognize these proteins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

Immunohistochemical analysis of paraffin-embedded human liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Flow Cytometry - Anti-KDEL antibody [EPR12668] (ab176333)

Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling KDEL (red) with purified ab176333 at dilution of 1/100. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

Immunohistochemical analysis of paraffin-embedded rat kidney sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-KDEL antibody [EPR12668] (ab176333)

All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/10000 dilution

Lane 1 : Raw264.7 whole cell lysate
Lane 2 : C6 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size : 94,78,57,48

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDEL antibody [EPR12668] (ab176333)

Immunohistochemical analysis of paraffin-embedded mouse liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Anti-KDEL antibody [EPR12668] (ab176333)

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