Golgi Staining Kit - Green Fluorescence - Cytopainter (ab139483)
Overview
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Product name
Golgi Staining Kit - Green Fluorescence - Cytopainter -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Golgi Staining Kit - Green Fluorescence | Cytopainter (ab139483) contains an Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, and human bone osteosarcoma epithelial cell line, U2OS. One important application of ab139483 is in fluorescence co-localization imaging with red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
ab139483 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
Review other dyes and kits for Golgi staining, or the live cell staining fluorescent dyes guide
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Notes
This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum, and is resistant to photo-bleaching, concentration quenching and photoconversion.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests 10X Assay Buffer 1 1 x 15ml 50X Assay Buffer 2 1 x 1.2ml Golgi Green Detection Reagent 1 x 50nmole Hoechst 33342 Nuclear Stain 1 x 50µl -
Research areas
Images
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Fluorescence MicroscopyGolgi Green Detection dye co-localizes with N-acetylgalactosaminyltransferase-2, fused to red fluorescent protein (RFP). (A) Golgi Green Detection dye, (B) HeLa cells expressing RFP-N-acetylgalactosaminyltransferase-2, (C) Hoechst 33342 staining, (D) composite image.
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Fluorescence excitation and emission spectraFluorescence excitation and emission spectra for Golgi Green Detection dye dye (panel A) and absorbance and fluorescent emission spectra for Hoechst 33342 dye (panel B). All spectra were determined in 1X Assay Solution.
