Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 18 antibody (ab24561)

Key features and details

  • Rabbit polyclonal to Cytokeratin 18
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cytokeratin 18 antibody
    See all Cytokeratin 18 primary antibodies

  • Description

    Rabbit polyclonal to Cytokeratin 18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat and A431 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab24561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • Tissue specificity

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • Involvement in disease

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (ab24561)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (ab24561)
    ICC/IF image of ab24561 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Western blot - Anti-Cytokeratin 18 antibody (ab24561)
    Western blot - Anti-Cytokeratin 18 antibody (ab24561)
    All lanes : Anti-Cytokeratin 18 antibody (ab24561) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Flow Cytometry - Anti-Cytokeratin 18 antibody (ab24561)
    Flow Cytometry - Anti-Cytokeratin 18 antibody (ab24561)
    Overlay histogram showing MCF7 cells stained with ab24561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24561, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunoprecipitation - Anti-Cytokeratin 18 antibody (ab24561)
    Immunoprecipitation - Anti-Cytokeratin 18 antibody (ab24561)
    Cytokeratin 18 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Cytokeratin 18 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24561.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 48kDa; Cytokeratin 18

References (5)

Publishing research using ab24561? Please let us know so that we can cite the reference in this datasheet.

ab24561 has been referenced in 5 publications.

  • Gagniac L  et al. Membrane expression of the estrogen receptor ERa is required for intercellular communications in the mammary epithelium. Development 147:N/A (2020). PubMed: 32098763
  • Hahn JM  et al. Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. PLoS One 14:e0213325 (2019). PubMed: 30835771
  • Gioeli D  et al. Development of a multicellular pancreatic tumor microenvironment system using patient-derived tumor cells. Lab Chip 19:1193-1204 (2019). PubMed: 30839006
  • Gong Z  et al. Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development. Cell Death Dis 10:440 (2019). PubMed: 31165736
  • Singh R  et al. Functional Analysis of Serially Expanded Human iPS Cell-Derived RPE Cultures. Invest Ophthalmol Vis Sci 54:6767-78 (2013). Human . PubMed: 24030465

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (ab24561)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (ab24561)
    ICC/IF image of ab24561 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab24561, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Western blot - Anti-Cytokeratin 18 antibody (ab24561)
    Western blot - Anti-Cytokeratin 18 antibody (ab24561)
    All lanes : Anti-Cytokeratin 18 antibody (ab24561) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
  • Flow Cytometry - Anti-Cytokeratin 18 antibody (ab24561)
    Flow Cytometry - Anti-Cytokeratin 18 antibody (ab24561)
    Overlay histogram showing MCF7 cells stained with ab24561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24561, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunoprecipitation - Anti-Cytokeratin 18 antibody (ab24561)
    Immunoprecipitation - Anti-Cytokeratin 18 antibody (ab24561)
    Cytokeratin 18 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Cytokeratin 18 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24561.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 48kDa; Cytokeratin 18

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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