Anti-AKAP 95 antibody (ab72196)
Key features and details
- Rabbit polyclonal to AKAP 95
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AKAP 95 antibody
See all AKAP 95 primary antibodies -
Description
Rabbit polyclonal to AKAP 95 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human AKAP 95 aa 642-692 (C terminal).
Database link: NP_005849.1 -
Positive control
- HeLa and 293T whole cell lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKAP 95 antibody (ab72196)All lanes : Anti-AKAP 95 antibody (ab72196) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody (ab72196)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling AKAP 95 with ab72196 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-AKAP 95 antibody (ab72196)Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab72196 at 3 µg/mg lysate. 20% of the immunoprecipitate was used for Western blot and bands were detected using ab72196 at 1 µg/ml. Bands were developed using chemiluminescence with an exposure time of 3 seconds.
Lane 2 represents a Control IgG.
