Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8978(B)] to AKAP 95
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-AKAP 95 antibody [EPR8978(B)]
    See all AKAP 95 primary antibodies

  • Description

    Rabbit monoclonal [EPR8978(B)] to AKAP 95

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AKAP 95 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, BxPC-3, Jurkat, HepG2, HeLa and Fetal liver lysates. Human tonsil and liver tissue. HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C.

  • Dissociation constant (KD)

    KD = 1.29 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8978(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Western blot - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    All lanes : Anti-AKAP 95 antibody [EPR8978(B)] (ab134923) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : BxPC3 lysate
    Lane 3 : Jurkat lysate
    Lane 4 : HepG2 lysate
    Lane 5 : HeLa lysate
    Lane 6 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 76 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue staining AKAP 95 with ab134923 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunohistochemical analysis of paraffin-embedded Human liver tonsil staining AKAP 95 with ab134923 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunocytochemistry/ Immunofluorescence - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunofluorescence analysis of HepG2 cells staining AKAP 95 with ab134923 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunohistochemical analysis using ab134923 showing positive staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunohistochemical analysis using ab134923 showing positive staining in Normal heart tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

    Immunohistochemical analysis using ab134923 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    OI-RD Scanning - Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)
    Anti-AKAP 95 antibody [EPR8978(B)] (ab134923)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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