Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [H68.4] to Transferrin Receptor - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free
    See all Transferrin Receptor primary antibodies

  • Description

    Mouse monoclonal [H68.4] to Transferrin Receptor - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Transferrin Receptor. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P02786

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa and K562 whole cell lysate; Human, mouse and rat placenta lysate. IHC-P: Human placenta and cervical carcinoma tissue. ICC/IF: K-562 and RAW 264.7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269514 is the carrier-free version of ab269513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Western blot - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    All lanes : Anti-Transferrin Receptor antibody [H68.4] (ab269513) at 1/5000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Mouse placenta tissue lysate
    Lane 5 : Rat placenta tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 84 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Transferrin receptor can undergo glycosylation as shown in lane 4 and 5 (PMID: 30854239).

    Blocking/Diliution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1/3-5: 114 seconds; Lane 2: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulatio containing PBS, BSA, glycerol and sodium azide (ab269513).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human placenta. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Transferrin Receptor with ab269513 at 1/2000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cervical carcinoma. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Transferrin Receptor with ab269513 at 1/25 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269513).

  • Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)
    Anti-Transferrin Receptor antibody [H68.4] - BSA and Azide free (ab269514)

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