Albumin Creatinine Ratio Assay Kit (ab241018)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
Albumin Creatinine Ratio Assay Kit -
Detection method
Fluorescent -
Product overview
The Albumin Creatinine Ratio Assay Kit (ab241018) provides a simple, sensitive, and high throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/mL), creatinine (detection range: 0.002 -0.5 mg/mL) and Albumin-to-creatinine ratio.
The ACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Arcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Albumin Assay Buffer 1 x 7ml Albumin Diluent 1 x 7ml Albumin Probe (AB580) 1 x 0.4ml BSA Standard (2 mg/ml) 1 x 1ml Creatinase 1 vial Creatine Standard (10 µmol) (Lyophilized) Yellow 1 vial Creatininase 1 vial Creatinine Assay Buffer 1 x 25ml Creatinine Enzyme Mix 1 vial Creatinine Probe 1 x 0.2ml -
Research areas
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Cellular localization
Albumin: Secreted.
Images
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BSA Standard Curve.BSA Standard Curve.
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Creatinine Standard Curve.Creatinine Standard Curve. -
Estimation of ACR in human urine in diabetic (1) and non-diabetic donors (2, and 3).For Albumin, 50 µL of undiluted samples and for Creatinine, 30 µL of diluted samples (100 times diluted using Creatinine Assay Buffer) were assayed following the kit protocol.