Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
Suitable for: WB, ICC/IF, IP
Knockout validated
Reacts with: Human

Product name

Anti-PHD finger protein 6/PHF6 antibody [EPR11997]
See all PHD finger protein 6/PHF6 primary antibodies
Description

Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

within Human PHD finger protein 6/PHF6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IWS0
Positive control
- HeLa, A431, HEK-293, K562, and 293T cell lysates; HeLa cells.
General notes
This product was previously labelled as PHD finger protein 6

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11997
Isotype

IgG
Research areas

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : PHF6 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 41 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.
Immunoprecipitation - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab173304.
Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

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