Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PHD finger protein 6/PHF6 antibody [EPR11997]
    See all PHD finger protein 6/PHF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human PHD finger protein 6/PHF6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8IWS0

  • Positive control

    • HeLa, A431, HEK-293, K562, and 293T cell lysates; HeLa cells.

  • General notes

     

    This product was previously labelled as PHD finger protein 6

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11997

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : PHF6 knockout HEK-293 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 41 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

    Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.

  • Immunoprecipitation - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    Immunoprecipitation - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab173304.

  • Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
    Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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