Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)
![Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)](https://www.abcam.com/ps/products/170/ab170929/Images/ab170929-389673-anti-phd-finger-protein-6phf6-antibody-epr11996b-western-blot-human-lysate.jpg "Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11996(B)] to PHD finger protein 6/PHF6
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)]
See all PHD finger protein 6/PHF6 primary antibodies -
Description
Rabbit monoclonal [EPR11996(B)] to PHD finger protein 6/PHF6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human PHD finger protein 6/PHF6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8IWS0 -
Positive control
- WB: K562, HeLa, Jurkat and 293T cell lysates. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as PHD finger protein 6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11996(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PHF6 knockout HeLa cell lysate
Lane 3 : Wild-type HEK-293 cell lysate
Lane 4 : PHF6 knockout HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDaLanes 1-4: Merged signal (red and green). Green - ab170929 observed at 43 kDa. Red - loading control ab7291 observed at 50 kDa.
ab170929 Recombinant Anti-PHF6 antibody [EPR11996(B)] was shown to specifically react with PHF6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264902 (knockout cell lysate ab258110) was used. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. ab170929 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)](https://www.abcam.com/ps/products/170/ab170929/Images/ab170929-343306-Ab170929KOWBDOC.jpg)
Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/5000 dilution
Lane 1 : Wild-type HEK 293 whole cell lysate
Lane 2 : PHF6 knockout HEK 293 whole cell lysate
Lane 3 : Hela whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170929 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab170929 was shown to recognize PHD finger protein 6/PHF6 in wild-type HEK 293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab170929 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)](https://www.abcam.com/ps/products/170/ab170929/Images/ab170929-170900-anti-phd-finger-protein-6-antibody-epr11996b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)Immunofluorescence analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab170929 at 1/100 dilution.
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![Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)](https://www.abcam.com/ps/products/170/ab170929/Images/ab170929-170899-anti-phd-finger-protein-6-antibody-epr11996b-western-blot.jpg)
Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa
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![Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)](https://www.abcam.com/ps/products/170/ab170929/Images/ab170929-5-benefits-of-recombinant-antibodies.png)
Anti-PHD finger protein 6/PHF6 antibody [EPR11996(B)] (ab170929)
