Anti-Bcl-XL antibody (ab45002)
Key features and details
- Rabbit polyclonal to Bcl-XL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-XL antibody
See all Bcl-XL primary antibodies -
Description
Rabbit polyclonal to Bcl-XL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Bcl-XL aa 3-14 (N terminal).
Sequence:C-QSNRELVVDFLS
Database link: Q07817 -
Positive control
- Hodgkin's lymphoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcl-XL antibody (ab45002)All lanes : Anti-Bcl-XL antibody (ab45002) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated
Lane 2 : Hela Whole Cell Lysate - Staurosporine Treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 150 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)Human normal kidney cortex. Staining is localised to mitochondrial membranes and perinuclear envelope. Left panel: with primary antibody at 4 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplificatio
