Glucose Isomerase Activity Assay Kit (Colorimetric) (ab273289)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sensitivity: 50 µU
Overview
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Product name
Glucose Isomerase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Assay type
Enzyme activity (quantitative) -
Sensitivity
50 µU -
Assay duration
Multiple steps standard assay -
Product overview
Glucose Isomerase Activity Assay Kit (Colorimetric) (ab273289) provides a quick and easy method for monitoring Glucose Isomerase (GI) activity in various samples. For example, purified proteins and bacterial lysates (E. coli, Streptomyces murinus, etc.).
In this assay, GI converts Fructose into Glucose. This isomerization is detected through a series of enzymatic reactions generating an intermediate that reacts with the Probe producing a stable chromophore with strong absorbance at OD= 570 nm.
The assay is simple, sensitive and can detect GI activity as little as 50 µU in a variety of samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GI Assay Buffer 1 x 25ml GI Converter (Lyophilized) 1 vial GI Developer (Lyophilized) 1 vial GI Positive Control (in Ammonium Sulfate) 1 x 50µl GI Probe (in DMSO) 1 x 200µl GI Substrate 1 x 200µl Glucose Standard (100 mM) 1 x 100µl
Images
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Glucose standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Kinetic measurement of Glucose Isomerase activity from Streptomyces Lysates.Kinetic measurement of Glucose Isomerase activity from Streptomyces lysates (µg protein): Sample A (26 µg), Sample B (52 µg).
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Relative GI Activity was calculated in lysates prepared from E. coli. and Streptomyces.Assays were performed following kit protocol.