Anti-Bcl-2 antibody (ab194583)
Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-2 antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseRatHumanIHC-P MouseRatHumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Bcl-2 aa 1-100.
Database link: P10415 -
Positive control
- WB: HL-60 cell lysate, mouse testis, rat lung and rat liver tissue lysates; IHC: Human lung cancer, rat brain and mouse testis; ICC/ IF: C6, NIH-3T3 and U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab194583)
Paraffin-embedded human lung cancer tissue stained for Bcl-2 using ab194583 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab194583)Immunofluorescence staining of NIH-3T3 cells stained for Bcl-2 with ab194583 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-Bcl-2 antibody (ab194583)All lanes : Anti-Bcl-2 antibody (ab194583) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : Rat liver tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutesBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab194583)Paraffin-embedded mouse testis tissue stained for Bcl-2 using ab194583 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab194583)Immunofluorescence staining of U-2 OS cells stained for Bcl-2 with ab194583 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-Bcl-2 antibody (ab194583)Anti-Bcl-2 antibody (ab194583) at 1/500 dilution + HL-60 cell lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab194583)Paraffin-embedded rat brain tissue stained for Bcl-2 using ab194583 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab194583)Immunofluorescence staining of C6 cells stained for Bcl-2 with ab194583 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
