Anti-SCAP antibody (ab190103)
Key features and details
- Rabbit polyclonal to SCAP
- Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCAP antibody
See all SCAP primary antibodies -
Description
Rabbit polyclonal to SCAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SCAP aa 600-700 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q12770 -
Positive control
- Human breast and Human colon carcinoma tissues; HeLa and K562 cells; K562 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SCAP antibody (ab190103)Anti-SCAP antibody (ab190103) at 1/1000 dilution + K562 cell lysate at 35 µg
Predicted band size: 140 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (ab190103)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling SCAP with ab190103 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-SCAP antibody (ab190103)Immunofluorescent analysis of HeLa cells (4% PFA-fixed, 20min; Triton X-100 permeabilized, 0.2%, 30 min) labeling SCAP with ab190103 at 1/100 dilution for 2h RT. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAP antibody (ab190103)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SCAP with ab190103 at 5µg/ml dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
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Flow Cytometry - Anti-SCAP antibody (ab190103)Flow cytometric analysis of K562 cells labeling SCAP with ab190103 at 1/10 dilution (lower image) compared with a negative control (upper image). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
