Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y184] to PTEN - Low endotoxin, Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PTEN antibody [Y184] - Low endotoxin, Azide free
    See all PTEN primary antibodies

  • Description

    Rabbit monoclonal [Y184] to PTEN - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab157804)

  • Positive control

    • WB: HAP1, MCF7 and HEK-293 cell lysates; Human brain lysate; Mouse primary bone marrow derived macrophage whole cell lysate.

  • General notes

    ab219361 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

    This data was developed using ab32199, the same antibody clone in a different buffer formulation.

    Lanes 1 and 5: PTEN knockout HAP1 cell lysate (20 µg)

    Lanes 2 and 6: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Green signal from target - ab32199 observed at 47 kDa

    Lanes 3 and 4: Red signal from loading control - ab8245 observed at 37 kDa

    Lanes 5 and 6: Merged (red and green) signal.

    ab32199 was shown to specifically recognize PTEN in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab32199 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

    This data was developed using ab32199, the same antibody clone in a different buffer formulation.

    Lanes 1: PTEN knockout HAP1 cell lysate (20 µg)

    Lanes 2: Wild-type HAP1 cell lysate (20 µg)

    Green signal from target

    Red signal from loading control - ab8245 observed at 37 kDa

    This western blot image is a comparison between ab32199 and a competitor's top cited mouse monoclonal antibody.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    All lanes : Brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32199, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HEK293 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    This data was developed using ab32199, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (Unpurified) + MCF7 cell lysate

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    This data was developed using ab32199, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

    This data was developed using the same antibody clone in a different buffer formulation (ab32199).

    Lanes 1 and 5: PTEN knockout HAP1 cell lysate (20 µg)
    Lanes 2 and 6: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Green signal from target – ab32199 observed at 47 kDa
    Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab32199 was shown to specifically recognize PTEN in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab32199 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

    This data was developed using the same antibody clone in a different buffer formulation (ab32199).

    Lanes 1: PTEN knockout HAP1 cell lysate (20 µg)
    Lanes 2: Wild-type HAP1 cell lysate (20 µg)
    Green signal from target 
    Red signal from loading control – ab8245 observed at 37 kDa

    This western blot image is a comparison between ab32199 and a competitor's top cited mouse monoclonal antibody.

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    All lanes : Brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32199).

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HEK293 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32199).

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Western blot - Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (unpurified) + MCF7 cell lysate

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32199).

  • Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
    Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)

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