All lanes : Anti-SLC24A6 antibody (ab83551) at 1 µg/ml

Lane 1 : Human Placenta
Lane 2 : 293T cell lysate

Lysates/proteins at 25 µg per lane.

Predicted band size: 64 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Anti-SLC24A6 antibody (ab83551) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: 35 kDa, 95 kDa. We are unsure as to the identity of these extra bands.



Gel concentration 12%