Anti-KCC2 antibody [N1/12] (ab134300)
![Anti-KCC2 antibody [N1/12] (ab134300)](https://www.abcam.com/ps/products/134/ab134300/Images/ab134300-148649-anti-kcc2-antibody-n1-12-western-blot.jpg "Anti-KCC2 antibody [N1/12] (ab134300)")
Key features and details
- Mouse monoclonal [N1/12] to KCC2
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-KCC2 antibody [N1/12]
See all KCC2 primary antibodies -
Description
Mouse monoclonal [N1/12] to KCC2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Rat
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Immunogen
His-tag fusion protein, corresponding to amino acids 932-1043 of Rat KCC2
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Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S1-12 to N1/12, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N1/12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-KCC2 antibody [N1/12] (ab134300)Anti-KCC2 antibody [N1/12] (ab134300) at 1/1000 dilution + Rat membrane tissue lysate
Secondary
Standard goat anti-mouse IgG:HRP
Developed using the ECL technique.
Predicted band size: 126 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [N1/12] (ab134300)](https://www.abcam.com/ps/products/134/ab134300/Images/ab134300-311240-anti-kcc2-antibody-n1-12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KCC2 antibody [N1/12] (ab134300)4% formaldehyde-fixed SK-N-BE cells stained for KCC2 (green) using ab134300 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes room temperature, 5 minutes room temperature.
(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134300. (D) Composite.
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![Flow Cytometry - Anti-KCC2 antibody [N1/12] (ab134300)](https://www.abcam.com/ps/products/134/ab134300/Images/ab134300-204509-anti-kcc2-antibody-n1-12-flow-cytometry.jpg)
Flow Cytometry - Anti-KCC2 antibody [N1/12] (ab134300)Overlay histogram showing SH-SY5Y cells stained with ab134300 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134300, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
