Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R5E antibody [EPR17146] (ab198290)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17146] to PPP2R5E
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP2R5E antibody [EPR17146]
    See all PPP2R5E primary antibodies

  • Description

    Rabbit monoclonal [EPR17146] to PPP2R5E

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, Daudi, C6 and NIH/3T3 cell lysates; Human pancreas and fetal brain tissue lysate; Mouse and rat brain tissue lysates. ICC/IF: C6 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP2R5E knockout HeLa cell lysate
    Lane 3 : HEK-293 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Western blot - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor cell) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5E antibody [EPR17146] (ab198290)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor) cells labeling PPP2R5E with ab198290 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on C6 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198290 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Anti-PPP2R5E antibody [EPR17146] (ab198290)
    Anti-PPP2R5E antibody [EPR17146] (ab198290)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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