Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP229] to PTEN (phospho T366) - BSA and Azide free
  • Suitable for: Dot blot, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free
    See all PTEN primary antibodies

  • Description

    Rabbit monoclonal [EP229] to PTEN (phospho T366) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects PTEN phosphorylated on T366.

  • Tested applications

    Suitable for: Dot blot, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cervix adenocarcinoma treated with Calyculin A. IHC-P: Human breast ductal carcinoma tissue.

  • General notes

    Ab208104 is the carrier-free version of ab109454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab208104 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)
    Western blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)
    All lanes : Anti-PTEN (phospho T366) antibody [EP229] (ab109454) at 1/20000 dilution

    Lane 1 : Untreated HeLa whole cell lysate,10ug
    Lane 2 : HeLa treated with Calyculin A whole cell lysate,10ug
    Lane 3 : HeLa whole cell lysate treated with Calyculin A and phosphatase on the membrane,10ug

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa


    Exposure time: 1 minute


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109454).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109454).

    ab109454 at 1/100 dilution staining PTEN in Human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)
    Dot Blot - Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109454).

    Dot blot analysis of PTEN (pT366+ pS370) peptide (Lane 1), PTEN (pS370) peptide (Lane 2), PTEN (pT366) peptide (Lane 3), and PTEN non-phospho peptide (Lane 4) labelling PTEN phospho T366 with ab109454 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)
    Anti-PTEN (phospho T366) antibody [EP229] - BSA and Azide free (ab208104)

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