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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKA

Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11261] to AKAP3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-AKAP3 antibody [EPR11261] - BSA and Azide free
    See all AKAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11261] to AKAP3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249540 is the carrier-free version of ab170856.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11261
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA

Images

  • Western blot - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    Western blot - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    All lanes : Anti-AKAP3 antibody [EPR11261] (ab170856) at 1/1000 dilution

    Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
    Lane 2 : HuT-78 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 5 : NIH/3T3 cell lysate
    Lane 6 : NCI-H460 cell lysate
    Lane 7 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 95 kDa



    This data was developed using ab170856, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    Immunocytochemistry - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    This data was developed using ab170856, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling AKAP3 with ab170856 at 1/250 dilution (red). DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    Flow Cytometry - Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    This data was developed using ab170856, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling AKAP3 with ab170856 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)
    Anti-AKAP3 antibody [EPR11261] - BSA and Azide free (ab249540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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