Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5118-34] to Amyloid Precursor Protein - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR5118-34] to Amyloid Precursor Protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type HAP1, HepG2 and Hela whole cell lysate.

  • General notes

    Ab238916 is the carrier-free version of ab126732. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238916 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)
    Western blot - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: APP knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126732 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126732 was shown to specifically react with APP when APP knockout samples were used. Wild-type and APP knockout samples were subjected to SDS-PAGE. Ab126732 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126732).

  • Flow Cytometry - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)
    Flow Cytometry - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)
    Overlay histogram showing A431 cells stained with ab126732 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126732, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126732).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)

    ab126732, at a dilution of 1/100, staining Amyloid Precursor Protein in paraffin-embedded Human fetal brain tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126732).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)
    Anti-Amyloid Precursor Protein antibody [EPR5118-34] - BSA and Azide free (ab238916)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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