Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) (ab271787)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, DDDDK tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) -
Biological activity
Assay Conditions: ARV-771-mediated binding of VHL to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GSTBRD3 (BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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Purity
>= 80 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH -
Predicted molecular weight
13 kDa including tags -
Amino acids
2 to 108 -
Tags
His tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Sequence
PRRAENWDEA EVGAEEAGVE EYGPEEDGGE ESGAEESGPE ESGPEELGAE EEMEAGRPRP VLRSVNSREP SQVIFCNRSP RVVLPVWLNF DGEPQPYPTL PPGTGRRIHS YRGHLWLFRD AGTHDGLLVN QTELFVPSLN VDGQPIFANI TLPVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD -
Predicted molecular weight
25 kDa including tags -
Amino acids
2 to 212 -
Tags
DDDDK tag N-Terminus
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Amino Acid Sequence 3
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Species
Human -
Sequence
SLKPRVVDFD ETWNKLLTTI KAVVMLEYVE RATWNDRFSD IYALCVAYPE PLGERLYTET KIFLENHVRH LHKRVLESEE QVLVMYHRYW EEYSKGADYM DCLYRYLNTQ FIKKNKLTEA DLQYGYGGVD MNEPLMEIGE LALDMWRKLM VEPLQAILIR MLLREIKNDR GGEDPNQKVI HGVINSFVHV EQYKKKFPLK FYQEIFESPF LTETGEYYKQ EASNLLQESN CSQYMEKVLG RLKDEEIRCR KYLHPSSYTK VIHECQQRMV ADHLQFLHAE CHNIIRQEKK NDMANMYVLL RAVSTGLPHM IQELQNHIHD EGLRATSNLT QENMPTLFVE SVLEVHGKFV QLINTVLNGD QHFMSALDKA LTSVVNYREP KSVCKAPELL AKYCDNLLKK SAKGMTENEV EDRLTSFITV FKYIDDKDVF QKFYARMLAK RLIHGLSMSM DSEEAMINKL KQACGYEFTS KLHRMYTDMS VSADLNNKFN NFIKNQDTVI DLGISFQIYV LQAGAWPLTQ APSSTFAIPQ ELEKSVQMFE LFYSQHFSGR KLTWLHYLCT GEVKMNYLGK PYVAMVTTYQ MAVLLAFNNS ETVSYKELQD STQMNEKELT KTIKSLLDVK MINHDSEKED IDAESSFSLN MNFSSKRTKF KITTSMQKDT PQEMEQTRSA VDEDRKMYLQ AAIVRIMKAR KVLRHNALIQ EVISQSRARF NPSISMIKKC IEVLIDKQYI ERSQASADEY SYVA -
Predicted molecular weight
88 kDa including tags -
Amino acids
2 to 745 -
Tags
DDDDK tag N-Terminus
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Amino Acid Sequence 4
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Species
Human -
Sequence
DVFLMIRRHK TTIFTDAKES STVFELKRIV EGILKRPPDE QRLYKDDQLL DDGKTLGECG FTSQTARPQA PATVGLAFRA DDTFEALCIE PFSSPPELPD VMKPQDSGSS ANEQAVQ -
Predicted molecular weight
14 kDa including tags -
Amino acids
2 to 118 -
Tags
DDDDK tag N-Terminus
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Amino Acid Sequence 5
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Species
Human -
Sequence
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
Predicted molecular weight
13 kDa including tags -
Amino acids
2 to 112 -
Tags
His tag N-Terminus
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Description
Recombinant human Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged)
Preparation and Storage
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Amino Acid Sequence 5
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Species
Human -
Sequence
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
Predicted molecular weight
13 kDa including tags -
Amino acids
2 to 112 -
Tags
His tag N-Terminus
Images
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SDS-PAGE - Recombinant Human Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B protein (Tagged) (ab271787)
SDS-PAGE analysis of ab271787.
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Functional Studies - Recombinant Human Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B protein (Tagged) (ab271787)Functional studies of ab271787.