Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15843] to RRAS - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-RRAS antibody [EPR15843] - BSA and Azide free
    See all RRAS primary antibodies

  • Description

    Rabbit monoclonal [EPR15843] to RRAS - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251063 is the carrier-free version of ab191399. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251063 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    Western blot - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    All lanes : Anti-RRAS antibody [EPR15843] (ab191399) at 1/1000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : HT29 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This data was developed using ab191399, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    Western blot - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    Anti-RRAS antibody [EPR15843] (ab191399) at 1/10000 dilution + A549 cell lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This data was developed using ab191399, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    Flow Cytometry - Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    This data was developed using ab191399, the same antibody clone in a different buffer formulation.Flow cytometry analysis of paraformaldehyde-fixed A549 cells labeling RRAS with ab191399 at a 1/350 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
  • Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)
    Anti-RRAS antibody [EPR15843] - BSA and Azide free (ab251063)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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