Anti-Periostin antibody (ab14041)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Periostin antibody
See all Periostin primary antibodies -
Description
Rabbit polyclonal to Periostin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).
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Positive control
- IHC-P: Human colon and normal skin tissues; Prostate cancer tissues. ICC/IF: HeLa cells.
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General notes
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue sectionlabeling Periostin with ab14041. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041) Tian et al. PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).
(A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.
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Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab14041)ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
