Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Periostin antibody [EPR19936] (ab219057)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19936] to Periostin
Suitable for: IP, WB, IHC-P
Reacts with: Human

Product name

Anti-Periostin antibody [EPR19936]
See all Periostin primary antibodies
Description

Rabbit monoclonal [EPR19936] to Periostin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon cancer, stomach, breast cancer, fetal heart and fetal kidney lysates; HeLa whole cell lysate. IHC-P: Human colon and lung cancer tissues. IP: Human fetal heart lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19936
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] (ab219057)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219057 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID: 26023718).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] (ab219057)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219057 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID: 24273600).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Periostin antibody [EPR19936] (ab219057)

All lanes : Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1 : Human colon cancer lysate
Lane 2 : Human stomach lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Human breast cancer lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 93 kDa
Observed band size: 93 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-3: 30 seconds; Lane 4: 5 seconds.
Western blot - Anti-Periostin antibody [EPR19936] (ab219057)

All lanes : Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa
Observed band size: 93 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Periostin antibody [EPR19936] (ab219057)

Periostin was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab219057 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219057 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human fetal heart lysate 10 μg (Input).

Lane 2: ab219057 IP in Human fetal heart lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219057 in Human fetal heart lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Periostin antibody [EPR19936] (ab219057)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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