Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 16, 2021

Anti-TFPI antibody [EPR22977-16] (ab260041)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22977-16] to TFPI
Suitable for: WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-TFPI antibody [EPR22977-16]
See all TFPI primary antibodies
Description

Rabbit monoclonal [EPR22977-16] to TFPI
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human placenta lysate. HepG2, Caco-2, HUVEC and HT-29 lysates. Flow Cyt: HepG2 cells. IP: Human placenta lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22977-16
Isotype

IgG
Research areas

Western blot - Anti-TFPI antibody [EPR22977-16] (ab260041)

All lanes : Anti-TFPI antibody [EPR22977-16] (ab260041) at 1/1000 dilution

Lane 1 : Human placenta lysate
Lane 2 : Human colon lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 6 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 45,47 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22144186) .

Negative control: Human colon and LoVo (PMID: 20062932, 9311608).

Exposure times: Lanes 1-2:59 secs; Lane 3:3 secs; Lanes 4-5:15 secs; Lanes 6-7:59 secs.
Immunoprecipitation - Anti-TFPI antibody [EPR22977-16] (ab260041)

TFPI was immunoprecipitated from 0.35 mg human placenta lysate 10µg with ab260041 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260041 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human placenta lysate 10µg

Lane 2: ab260041 IP in human placenta lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260041 in human placenta lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Flow Cytometry - Anti-TFPI antibody [EPR22977-16] (ab260041)

Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling TFPI with ab260041 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Anti-TFPI antibody [EPR22977-16] (ab260041)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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